关键词:
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Algorithms
Brain Chemistry
Cytosol
Humans
Hydrogen-Ion Concentration
Magnesium
Mathematics
Occipital Lobe
Phosphocreatine
摘要:
Free cytosolic [Mg2+] can be assessed in vivo by P-31 MRS from the chemical shift of beta-ATP which in turn depends on the fraction of total ATP complexed to Mg2+ ions, The reliability of these in vive measurements depends on the availability of an appropriate in vitro calibration to determine the limits of chemical shifts of unbound ATP and Mg-ATP complexes, using solutions that mimic the in vive cytosolic conditions as far as possible, We used an algorithm and software to allow a quantitative definition of the Mg2+-binding molecules to build a semi-empirical equation that correlates the chemical shift of the beta-ATP signal to the [Mg2+] taking into account the amount of Mg2+ bound to all other constituents in solution, Our experiments resulted in a simple and reliable equation directly usable to assess in vive the free cytosolic magnesium concentration of human brain by P-31 MRS. Our method is also flexible enough to make it suitable for in vive measurements of [Mg2+] in other organs and tissues.