关键词： Algorithms   Applied mathematics   Bioassays and physiological analysis   Biology and life sciences   Cell biology   Cell signaling   Computational biology   Copy number variation   Gastroenterology and hepatology   Gene expression   Gene regulation   Genetic loci   Genetics   Genome analysis   Genome complexity   Genome-wide association studies   Genomics   Human genetics   Inflammatory bowel disease   MAPK signaling cascades   Mathematics   Medicine and health sciences   Microarrays   Physical sciences   Research and analysis methods   Research Article   Signal transduction   Signaling cascades   Simulation and modeling  

摘要： Background Inflammatory bowel disease (IBD) is an idiopathic, chronic disorder of unclear etiology with an underlying genetic predisposition. Recent genome-wide association studies have identified more than 200 IBD susceptibility loci, but the causes of IBD remain poorly defined. We hypothesized that rare (<0.1% population frequency) gene copy number variations (CNVs) could play an important mechanism for risk of IBD. We aimed to examine changes in DNA copy number in a population-based cohort of patients with IBD and search for novel genetic risk factors for IBD. Methods DNA samples from 243 individuals with IBD from the Manitoba IBD Cohort Study and 2988 healthy controls were analyzed using genome-wide SNP microarray technology. Three CNV calling algorithms were applied to maximize sensitivity and specificity of CNV detection. We identified IBD-associated genes affected by rare CNV from comparing the number of overlapping CNVs in IBD samples with the number of overlapping CNVs in controls for each gene. Results 4,402 CNVs detected by two or three algorithms intersected 7,061 genes, in at least one analyzed sample. Four genes (e.g. DUSP22 and IP6K3) intersected by rare deletions and fourteen genes (e.g. SLC25A10, PSPN, GTF2F1) intersected by rare duplications demonstrated significant association with IBD (FDR-adjusted p-value < 0.01). Of these, ten genes were functionally related to immune response and intracellular signalling pathways. Some of these genes were also identified in other IBD related genome-wide association studies. These suggested that the identified genes may play a role in the risk of IBD. Conclusion Our results revealed new genomic loci associated with IBD, which suggested the role of rare CNVs in IBD risk.

关键词： Ellagitannins   Pomegranate juice   Punica granatum L.   Inflammation   Fecal calprotectin   IBD   Inflammatory bowel disease   Crohn's disease   Ulcerative colitis  

摘要： Background: Inflammatory bowel disease (IBD) is a chronic condition characterized by recurrent episodes of intestinal inflammation and is thought to be related to an autoimmune reaction to genetic and environmental factors. Although evidence indicates that a polyphenolic-rich diet plays an important role in modulating aspects of chronic inflammation, few studies have focused on the effect of ellagitannin (ET)-rich food consumption on longterm remission maintenance in IBD patients with a high risk of clinical relapse. Therefore, we hypothesize that supplementation with a pomegranate juice, a naturally rich source of ETs, could significantly modulate the markers of mucosal and systemic inflammation relative to a control group receiving a placebo. Methods/design: This double-blind, randomized controlled trial includes patients with IBD involving the colorectum who have been in stable therapy for at least the three previous months and have a high risk of clinical relapse. Participants are randomly allocated to one of two groups: active supplementation (125 mL of cv. Wonderful pomegranate juice) or placebo (125 mL) taken twice daily for 12 weeks. The primary outcome is changes in the fecal neutrophil-derived protein calprotectin, a surrogate marker of mucosal improvement, between the two groups from baseline to 12 weeks later. The secondary outcomes include transcriptomic changes in peripheral blood mononuclear cells and intestinal biopsies and changes in circulating inflammatory markers and trimethylamine-N-oxide levels. Pomegranate ET-derived metabolites are identified and quantified in plasma and urine samples. Discussion: The results will provide information on the possible reduction of fecal calprotectin levels following the consumption of pomegranate juice. The findings will also show the in vivo metabolism of pomegranate ETs. Finally, the effect of 12-week pomegranate juice consumption on local and systemic inflammatory markers will be elucidated, which will likel

关键词： 肛肠疾病   术后疼痛   中医干预  

摘要： 目的观察中医干预措施对肛肠疾病术后疼痛的影响。方法选择我院肛肠疾病行手术治疗的患者90例,将患者随机分成对照组(采用常规护理)与观察组(采用常规护理和中医干预措施)两组,每组45例。观察两组患者的术后疼痛情况、焦虑抑郁情绪和生活质量情况。结果治疗后和对照组相比,观察组患者疼痛评分(NRS)显著降低(P<0.05);治疗后和对照组相比,观察组患者焦虑自评量表评分(SAS)、抑郁自评量表评分(SDS)评分均显著降低(P<0.05);和治疗前相比,治疗后两组患者的SAS、SDS评分均显著降低(P<0.05)。治疗后和对照组相比,观察组患者SF-36量表评分显著升高(P<0.05)。结论中医干预能够显著降低肛肠疾病患者术后疼痛,缓解患者的不良情绪,提高患者的生活质量。

关键词： 炎性肠疾病/流行病学   炎性肠疾病/病理生理学   炎性肠疾病/诊断   炎性肠疾病/治疗   会议  

摘要： 一年一度的国际消化领域盛会——美国消化系统疾病周(DDW)于2019年5月18—21日在美国圣地亚哥举办。DDW是世界上最大和最负盛名的胃肠道疾病领域的专业会议,自1969年由美国肝病研究学会、美国胃肠病学会、美国胃肠内窥镜学会和消化道外科学会联合主办以来,至今已经走过了50个年头,每年都会有超过15 000名来自全球的消化领域杰出医师、学者参加,共同就胃肠病学、肝病学、内镜学和胃肠外科等领域的最新进展进行深入探讨。

关键词： 地奥司明   肛肠疾病   创缘水肿  

摘要： 目的:对肛肠疾病术后创缘水肿治疗中给予地奥司明用药取得的疗效进行观察。方法:选取广州中医药大学附属第一医院2018年1月至6月行肛肠疾病术后创缘水肿患者39例,采用术后常规换药方法,纳入对照组;另取本院2018年7月至12月行肛肠疾病术后创缘水肿患者39例,常规换药配合地奥司明用药,纳入观察组,对两组患者用药后取得的治疗效果观察。结果:治疗前,两组患者均有明显的水肿症状,评分结果相近,差异无统计学意义(P>0.05);观察组治疗后7d、14d的评分结果均较对照组更低,组间比较,差异均具有统计学意义(P<0.05)。观察组患者水肿症状消退时间为(6.00±1.50)d,短于对照组的(9.50±1.00)d,组间比较,差异具有统计学意义(P<0.05)。结论:肛肠疾病术后创缘水肿患者临床治疗中,常规用药配合地奥司明治疗,可取得显著疗效,临床症状改善明显。

关键词： 肛肠术后护理   中药坐浴   微波理疗  

摘要： 目的分析中药坐浴配合微波理疗在肛肠疾病患者术后护理中的应用价值。方法选取医院肛肠科收治的患者共计100例,随机分为对照组和观察组,每组50例。观察组采用中药坐浴配合微波理疗的护理干预方法,对照组采取常规基础护理,比较2组临床护理疗效以及护理前后VAS疼痛评分。结果观察组护理总有效率为80. 00%,高于对照组的60. 00%(P <0. 05);护理后,2组VAS疼痛评分较护理前均下降,且观察组下降幅度大于对照组(P均<0. 01)。结论肛肠术后患者采用中药坐浴配合微波理疗能够有效提高患者的护理效果,减轻术后的疼痛感,具有临床推广价值。

关键词： 疾病检查   肛肠科   肛门直肠   医院检查   常规检查   肛门周围   肛门镜   小叶  

摘要： 小叶有一个难言之隐,那就是患了肛门直肠瘘(简称肛瘘),肛门常常流脓,让他苦不堪言。可他不好意思面对医生,也担心到医院做检查太麻烦,便一直没有就医。其实,肛肠科的检査并没有小叶想象的那样复杂,常规检查有视诊、指诊和肛门镜。一旦发现自己的肛门周围长了东西,或小腹及肛门有下坠、便血、流脓等情况,千万不要耽误,尽快去医院检查治疗。