关键词:
Anthocyanins
Anti-inflammatory
Anti-oxidant
Co-cultures
Inflammatory bowel disease
摘要:
The overproduction of reactive oxygen species (ROS) and pro-inflammatory mediators is closely related with inflammatory diseases, such as inflammatory bowel disease (IBD). Despite the advances in therapeutics, new and safer strategies are still required. In this context, anthocyanins, highly available in human diet and recognized as health-promoting agents, have been a focus of interest. Thus, the aim of this work was to study the anti-inflammatory potential of a characterized anthocyanin-rich fraction from Portuguese blueberries, as compared with 5-aminosalicylic acid (ASA), a standard anti-inflammatory drug in IBD, by using a co-culture system of intestinal epithelial Caco-2 cells and RAW 264.7 macrophage cells, as a model of colitis. The compounds were pre-incubated in the apical side of a Transwell plate containing a differentiated and polarized Caco-2 cell monolayer, for 3 h, followed by addition of 1 µg/mL lipopolysaccharide to the basolateral side to stimulate the RAW 264.7 cells. After 24 h, the inflammatory process was evaluated in terms of nitric oxide, IL-6 and IL-1β contents in culture supernatants, as well as of intracellular ROS. A monoculture of RAW 264.7 was used for comparison. Our data showed that the production of pro-inflammatory mediators and ROS in both compartments was significantly decreased by anthocyanins, in a higher extent than by 5-ASA. [ABSTRACT FROM AUTHOR]