关键词： Infectious Disease   Hela Cell  

关键词： Blood Sample   Infectious Disease   Heparin   Tissue Culture   HeLa Cell  

摘要： Using horse serum adapted HeLa cells tissue culture neutralization tests have been carried out with 0.1 ml whole blood samples diluted in 0.2 ml heparin solution as test material. Good agreement was in general found between these tests and standard tests, but titers obtained with the small blood samples seemed to be higher than the ones obtained by the usual method. © 1958 Springer-Verlag.

关键词： Tissue Culture   Poliomyelitis   Poliomyelitis Virus  

关键词： Enzyme   Enzyme Activity   Colchicin   Poliomyelitis   Poliomyelitis Virus  

摘要： The effects of colchicine on polio virus growth in Maithland type tissue cultures of Rhesus kidney are described. Colchicine did not interfere with virus synthesis although it did affect specific nucleotidase and nuclease systems.

摘要： Die bei der Lebendbeobachtung und im gefärbten Präparat gemachte Feststellung eines unterschiedlichen Ablaufes im cytopathogenen Effekt zwischen Poliomyelitisvirus Typ 1 und ECHO-Virus Typ 9-Infektionen konnte in der Plaquegestalt bei Anwendung einer Zellzüchtung unter Agar nachDulbeccoundVogt3, 4in der Modifikation vonHsiungundMelnick9wiedererkannt werden. Das ECHO-Virus Typ 9 läßt im Gegensatz zum Poliomyelitisvirus Typ 1 gesunde lebande Zellen in schon sehr fortgeschrittenen Degenerationsherden zu, die ersten Plaques erscheinen im allgemeinen bei ECHO-Virus 9, Stamm K 696, etwas später, und sie nehmen nicht so schnell an Größe zu (Tabelle 2). Neben den bekannten, von anderen Autoren beobachteten Unterschieden in der Plaquebildung zeigten sich Unterschiede innerhalb eines ECHO-Virustyps, und zwar zwischen unserem Epidemiestamm des Jahres 1956 K 696 und dem vonMelnickbeschriebenen ECHO-Virus 9-Stamm (Quigley). Diese Beobachtungen unterstreichen die Bedeutung der Plaquemethode für die Differenzierung und Charakterisierung von Ent

摘要： Zone electro-phoresis on type I polio virus showed that the infectivity is associated with at least 2 components of different mobilities. Type I poliomyelitis virus was separated by zone electrophoresis in a density gradient into 2 infective fractions, of which the faster migrating one preserves its identity on re-electrophoresis. Density-gradient electrophoresis appears promising as a method for purifying viruses grown in tissue culture.

关键词： Viruses   Mice   Poliomyelitis   Adult insects   Snails   Cockroaches   Disease transmission   Nymphs   Feces  

摘要： In a search for a living invertebrate species that might serve in the capacity of a culture vehicle and medium for different viruses, mouse poliomyelitis virus strain FA having a 50% endpoint of 10-5 was injected into the hemocoeles of several species of invertebrates, and at intervals thereafter suspensions of their ground bodies were titrated in mice to determine levels of virus titer. In all instances the titer dropped steadily from the highest of just under 10-5 immediately after injection to less than 10-l near the end of the survival period. Maximum survival times were: Lymnaea stagnalis (L.), a snail, 7 days;Biom-phalaria (Australorbis) glabratus (Say), a snail, 14 days;Acheta assimilis F., a cricket, 21 days;Rhodnius prolixus Stal, a conenose bug, 21 days;Triatoma phyllosoma pallidipennls (Stal), a conenose bug, 28 days;and Triatoma infestans (Klug), a conenose bug, 21 days. Certain implications are drawn as to possible use of living invertebrates as culture vehicles for viruses and as to possible roles in survival and, potentially, dissemination of human poliomyelitis virus.

关键词： Inactivated poliovirus vaccines   Polio   Antibodies   Poliovirus   Infections   Globulins   Poliovirus vaccines   Blood   Spinal cord  

摘要： Like other stories about the control of an infectious disease, this tells of the years of research, the trials and errors, the frustrations and hopes, and finally what is believed to be success.(C) Lippincott-Raven Publishers.

关键词： Inactivated poliovirus vaccines   Poliomyelitis   Registered nurses