关键词： Infectious Disease   Reversion Rate   Significant Trend   Virus Isolate   General Tendency  

摘要： The d character appeared to be strongly temperature-dependent and, conversely, the rct character was affected by the sodium bicarbonate concentration. The extent of these interactions seemed to be a strain characteristic. Consequently, the importance of maintaining a constant and well-defined temperature of incubation during these genetic marker tests is stressed.

关键词： Infectious Disease   Genetic Marker   Guanidine   Parental Strain   Kidney Cell  

摘要： Following mixed infection of monkey kidney cells with a guanidine-sensitive clonal derivative of the attenuated LSc2ab type 1 poliovirus strain and with a guanidine-resistant clonal derivative of the MEF1 type 2 strain, the frequency of guanidine-resistant type 1 particles was found to be increased in the progeny. The frequency of these particles was almost 4 times higher than that of sponataneous mutants in the progeny of the self cross,, This statistically significant difference favours the occurrence of recombination between the two serologically unrelated poliovirus strains. Investigation of a group of unselected genetic markers in the type 1 guanidine-resistant clonal strains derived from the progeny of mixed infection showed that transfer of guanidine resistance from the type 2 parental strain was not associated with the transfer of any other character of this strain.

关键词： 脊髓灰质炎   小儿麻痹   肠道病毒感染   病毒疫苗   麻疹减毒疫苗   联合应用   球蛋白   口服疫苗  

摘要： 脊髓灰质炎口服疫苗和球蛋白缓和的麻疹减毒疫苗的分别使用,其有效率约在90%以上.但当两种病毒疫苗联合应用时,是否病毒间会产生干扰?是否球蛋白能抑制脊髓灰质炎病毒的免疫性?因此,对这两种病毒疫苗的联合应用进行免疫效果的评价是必需的. 作者选择了未服过脊髓灰质炎疫苗,亦没有麻疹病史的9个月到6岁的儿童,每个儿童口服三滴单价Ⅰ型或Ⅱ型脊髓灰质炎疫苗(200000-500000TCID50),有15个儿童口服Ⅰ型疫苗,8个服Ⅱ型疫苗.这23个儿童也接受皮下注射减毒麻疹疫苗0.5毫升(1000TCID50),同时在对侧三角肌区注入0.01毫升/磅体重人的免疫血清球蛋白.每个儿童在接种前采血标本.服Ⅰ型疫苗者在服后21～23天,服Ⅱ型疫苗者在服后16和44天,分别采血送检.脊髓灰质炎的抗体水平是以中和试验（变色法）确定的;麻疹的补体结合抗体和血凝抑制抗体也分别予以测定.接种前后的血清是同时测定的. 在口服Ⅰ型或Ⅱ型脊髓灰质炎疫苗的23个儿童

摘要： Attenuated polioviruses grown in hyman diploid cell strains have the same properties as the same virus strains grown in monkey kidney tissue. Such vaccines may be administered immediately after birth without risk of exposure of the newborn infant to virus contaminants arising in simian tissue.

关键词： Virology   Medical Microbiology   Infectious Diseases  

摘要： Résumé La caractérisation des souches de poliovirus isolées dans les suspensions fécales d'enfants atteints de poliomyélite paralytique et d'enfants vaccinés avec le vaccin vivant a été effectuée grâce à deux techniques de marquage: le test rct/40 et le test de serodifférenciation intratypique (techniqueMac Bride).La précision, la valeur et les limites de ces techniques ont été abordées en détail, en particulier lorsqu'il s'agit d'étudier les souches virales dans le cas de vaccinations effectuées dans un milieu déjà infecté par un poliovirus *** études ont montré la grande stabilité antigénique des souches de poliovirus sauvage. Il semble donc qu'au cours des vaccinations effectuées en période épidémique la meilleure méthode pour différencier les souches de poliovirus, sauvage et vaccinal, consiste à effectuer des sérodifférenciations intratypiques croisées avec deux antisérums spécifiques l'un de la souche épidémique, l'autre de la souche vaccinale.

关键词： 脊髓灰质炎减毒活疫苗   中和抗体   血清学反应   单价  

摘要： 口服脊髓灰质炎减毒活疫苗在广州还是第一次,为了了解这次口服活疫苗的效价,除了结合流行病学进行远期观察外,进行了血清学反应的分析,我们选择了5个托儿所采集血清标本。口服活疫苗方法分为两种,第1种Ⅰ、

关键词： 脑膜炎球菌   流行性脑脊髓膜炎   流脑   小儿麻痹病毒   肠道病毒   脊髓灰质炎病毒   肺炎球菌脑膜炎  

摘要： 脑腊炎是中枢神经系统感染的常见疾患,可由多种化脓性或非化脓性病原引起,临床表现虽类似,但治疗措施及转归殊异,因此如何提高认识,做到正确诊断、及时治疗以降低病死率、减少并发症仍是一重要课题。诊断的建立:在考虑脑膜炎的诊断时,应

关键词： Kidneys   Tissue culture techniques   Tuberculosis vaccine   Tissue procurement   Oral poliovirus vaccines   Monkeys   Test systems   Poliovirus  

摘要： The results of testing oral poliovirus vaccine produced over a four-year period are reported. Fifty-six per cent of the vaccine harvests produced in rhesus monkey kidney tissue culture and 36% of the vaccine harvests produced in cercopithecus monkey kidney tissue culture were rejected because of adventitious viruses. Simian foamy virus, simian measles virus and SV-40 were the most common viruses found in rejected vaccine harvests produced from rhesus monkey kidney tissue culture. Simian foamy virus and simian measles virus were the most common viruses found in cercopithecus monkey kidney tissue culture. The isolation of adventitious agents in various test systems is discussed.

关键词： 呼吸道   传染病   传染性疾病   疾病   病毒种类   肠道病毒   脊髓灰质炎病毒   小儿麻痹病毒   副流感   副流行性感冒   腺病毒  

摘要： 一、腸道和呼吸道病毒的种类: 在过去数年中,由于乳鼠尤其是組織培养技术的广泛应用,分出的腸道和呼吸道病毒很多,而且新的病毒还在继續发现,因此旧的病毒又可能归并或改属至其他病毒組內,这样,就引起了病毒分类和命名的困难。Evans共列举了124种腸道和呼吸道病毒(表1)。Hilleman1963年提出的呼吸道病毒分类法,其中也包括腸道病毒(表2)。从表1、2来看,就可知腸道和呼吸道病毒种类繁多,命名复杂,其中有一些是最近几年才发现的,故择要略加介紹。

关键词： Infectious Disease   Antibody Response   Antibody Titre   Vaccine Virus   Virus Isolate  

摘要： The new attenuated type 3 poliovirus, denoted as USOL-D bac virus, was administered to a group of children, at least nine of them having been fed earlier withSabin's vaccine. The data obtained in tests on virus excretion, antibody response and genetic stability of the virus indicate: 1. The virus multiplied well in the alimentary tract of children without preexisting homotypic antibodies and also in those who possessed antibodies resulting from previous feedings withSabin's vaccine. 2. All children who excreted the virus responded with development or marked enhancement of type 3 antibody titres. Increase of type 3 antibodies was also detected in one child from which the vaccine virus was not isolated and in the infected contact child. 3. An alteration in rct./40− character was observed only in five of 26 virus isolates tested. Ten of them were inoculated intracerebrally to 20 monkeys. No monkey was paralysed and only two animals developed slight muscular weakness. This seems to indicate a relatively high degree of genetic stability of the USOL-D bac virus during human passage.