关键词： Secondary Structure   Chain Length   Spermine   Short Chain   Ribonucleic Acid  

摘要： The presence of spermine during heating and quick cooling of the infectious RNA of Sindbis virus increased the survival 11-fold;a 4-fold increase was obtained with diaminohexane;no protection was afforded by amines of shortest chain length. None of the amines tested protected poliovirus RNA against heat. The absorbance-temperature profiles were obtained for RNA''s extracted from the purified viruses. A sharp hyperchromic effect was shown by Sindbis RNA but not by polio RNA. The increase in optical density was 32% and the transition midpoint 65.5''C. These figures are higher than those published for other single stranded RNA viruses and contrast with the fact that Sindbis virus has not the highest G+C content. The infectivity of Sindbis RNA was less susceptible to the action of pancreatic ribonuclease than was the infectivity of polio RNA. Sindbis RNA, in contrast to polio RNA, has a secondary structure, and this structure is necessary for the infectivity.

关键词： Clinical Trial   Infectious Disease   Vaccine Strain   Large Clinical Trial   Small Clinical Trial  

摘要： A small clinical trial with the Glenn type 3 poliovirus vaccine showed that the strain is antigenic and retains its attenuated characters on human passage at least as well as any other reported type 3 strain. The antigenicity and stability of the various type 3 vaccine strains should be compared in a larger clinical trial.

关键词： Science   Humanities and Social Sciences   multidisciplinary  

摘要： THE use of type 1 poliovirus mutants resistant to dextran sulphate, as described by Takemoto and Liebhaber1 and Takemoto and Kirschstein2, has proved extremely promising for genetic investigations. We have therefore carried out some additional investigations of these mutants with the view of using them in recombination experiments.

关键词： Sucrose   Infectious Disease   Inhibitory Activity   Specific Binding   Binding Capacity  

摘要： Inhibitors found in certain equine sera active against poliovirus type 1 were determined by the gel-adsorption method using aluminium-hydroxide-gel in the absence of cells. The inhibitor belongs to the 19 S-class of macroglobulins (IgM), as revealed by gel-filtration with Sephadex G 200, by DEAE-cellulose-chromatography, and by sucrose density centrifugation. It was bound to the viral surface in-vitro in the absence of tissue cells. The specific complex may be precipitated with anti-equine globulin from rabbits. The inhibitor was destroyed by papain and by 2-mercapto-ethanol. The "residual infectivity" (10-25%) was bound to the inhibitor in-vitro. Its behavior in the gel-adsorption system was not altered if the virus-inhibitor complexes were previously diluted. Nonsensitive mutants and double mutants were selected. There must exist at least 3 different combining sites for equine inhibitors on the surface of poliovirus type 1, strain Mahoney. Equine sera may be grouped according to the specificity of their inhibitory activity. The specific binding capacity was lost if the virus is heated at 50[degree] C for 30 min.

关键词： 脊髓灰质炎病毒   患者   型别   小儿麻痹病毒   肠道病毒   广州市   致病病毒   顾方舟  

摘要： 为了证实和了解脊髓灰质炎病毒在广州地区的流行情况和型别,我们在本地区收集了市儿童医院、市传染病院疑似的脊髓灰质炎患者的大便标本共181份,采用细胞培养方法分离与鉴定病毒,现将所得结果报告如下:材料与方法1.患者大便的收集及处理:收集经临床诊断为小儿麻痹症病人大便放在链霉素瓶内由冰壶运送至本实

摘要： A sample group of children who had no poliovirus antibody before vaccination was tested three years after vaccination. For poliovirus Types II and III, 100 per cent and 94.5 per cent of the children, respectively, retained antibody titers of at least 116. Only for Type I was the antibody-positive rate appreciably lower than at two years after vaccination, 81.3 per cent compared with 94.5 per cent;eight more children had a titer below 1:16. However, all but two children had a Type I titer of at least 1:8.

关键词： 活疫苗   婴幼儿   广州   广东   服用率   小儿麻痹   肠道病毒感染   脊髓灰质炎   保护率   反应   效果观察  

摘要： 脊髓灰质炎口服活疫苗为预防脊髓灰质炎的有效制剂,已为世界各国所公认。我省自1961—1963年连续3年在广州、佛山、江门、韶关、湛江、海口等市推广使用了国产脊髓灰质炎口服活疫苗(以下简称活疫苗),并进行了

关键词： Science   Humanities and Social Sciences   multidisciplinary  

摘要： VARIATION in the chemical properties of the protein coat of different strains has been used as a marker test in investigations of the genetics of polioviruses. Bengtsson, Philipson, Persson and Laurent1 recently showed that a group of these tests had a common basis. Small differences in the electrical charge of the protein of various strains of the same serotype resulted in different patterns of binding to charged adsorbents.

关键词： 毒株   沉淀反应   琼脂   抗原性   脊髓灰质炎病毒   小儿麻痹病毒   肠道病毒   免疫血清   病毒悬液  

摘要： 琼脂弥散沉淀试验曾用于流行性感昌病毒、痘类病毒、脊髓灰质炎病毒（以下简称灰质炎病毒）、腺病毒和某些肠道病毒的研究。本文报告我们对灰质炎病毒的沉淀抗原的制备、试验的技术方法及強毒株和減毒株的抗原性进行研究的结果。材料和方法一、病毒株所用病毒株包括Ⅰ型（Mahoney）、Ⅱ型（MEF2）和Ⅲ型（Saukett）灰质炎病毒原型株及Ⅰ型（***.2ab）、Ⅱ型

关键词： Tissue Culture   Virus Strain   Intestinal Tract   Vaccine Virus   Kidney Cell  

摘要： A description is given of a simple method for the intratypic serodifferentiation of polio type 1 viruses, based on a modified determination of neutralization index with specific anti-LSc-2ab sera. The principal modification is a very short incubation of the virus-serum mixtures before they are inoculated into tissue cultures of monkey kidney cells. The results of this method agree with those of the Wecker technique. The method has been used to study the antigenic character of virus strains excreted after oral vaccination. The antigenic marker is not stable during the intestinal passage of the virus. In the majority of cases the isolated strains gradually lose their antigenic relationship to the parent virus. The temperature marker has proved to be relatively stable. No correlation could be found between anti-genic and temperature marker. During multiplication of the virus in the intestinal tract a slight increase of monkey neurovirulence may occur, which is, however, not correlated with the loss of antigenic relationship to the vaccine virus. The applicability of the method is restricted because no differentiation can be made between a natural wild strain and a vaccine-derived strain which has lost its relation-ship to the parent virus.