关键词： 病毒性脑膜炎   脑脊液   细胞数   脊髓灰质炎病毒   小儿麻痹病毒   肠道病毒   病毒病  

摘要： 近年来,随着抗菌素的普遍使用,化脓性脑膜炎大大减少,病毒性脑膜炎特别是肠道病毒引起的脑膜炎,在儿科临床上占有重要地位。Wallgren根据疾病早期的脑膜刺激症状,脑脊液细胞数增多,镜检和培养不能证明脑脊液中有细菌存在等理由,提出“无菌性脑膜炎”的名称。其后随着临床检验技术的进步和临床病毒学的发展,“无菌性脑膜炎”的病因先后阐明,故有人认为无菌性脑膜炎的名称是不妥当的,建议用“非化脓性脑膜炎”代替,其中由病毒作

摘要： Patients previously described with cartilage-hair hypoplasia, a distinctive form of short-limbed dwarfism, havebeen found to have deficient cell-mediated immunity with intact antibody-mediated immunity. The patient with cartilage-hair hypoplasia described in the present report is unusual in that she had both deficient antibody-mediated immunity and deficient cell-mediated immunity. In addition, she developed severe, vaccine-related paralytic poliomyelitis. This complication suggests that live viral vaccines should not be administered to children with short-limbed dwarfism until the form of short-limbed dwarfism is established and immunologic evaluation is performed when indicated.

关键词： Carbon, activated   Organics, dissolved   Virus removal   Wastewater  

摘要： The effect of pH and soluble organic matter on virus adsorption to activated carbon in treated sewage was determined. Poliovirus removal from wastewater effluent was greatly improved by lowering the pH to 3.5-4.5 or by reducing the amount of organics by lime coagulation. Batch studies indicated that virus adsorption to activated carbon in wastewater could be described by Freundlich isotherms. In column experiments virus removal was found to be dependent on column length as well as hydraulic loading. Virus and soluble organics adsorbed at low pH could become deadsorbed by a rise in pH. © 1975, American Chemical Society. All rights reserved.

关键词： Infectious Disease   HeLa Cell   Indirect Evidence   Phosphodiesterase   Phosphorylase  

摘要： The infectivity of replicative form RNA (RF-RNA) isolated from poliovirus-infected HeLa cells is completely resistant to the action of T1 RNase but decreases after exposure to RNase A in the presence of 0.3 M NaCl. Under these conditions neither enzyme produces single-stranded nicks in RF-RNA. Three endonucleasefree exonucleases (RNase II, polynucleotide phosphorylase and spleen phosphodiesterase) rapidly destroy the infectivity of single-stranded RNA, but do not alter the infectivity of *** is concluded that RF-RNA does not contain single-stranded ends essential for infectivity. Indirect evidence suggests that all or most of the poly A region at the 3′ end of the plus strand of infectious RF-RNA is base-paired to a poly U region at the 5′ end of the minus strand.

关键词： 脊髓灰质炎病毒   小儿麻痹病毒   肠道病毒   抗体效价   疫苗免疫  

摘要： 1964年作者先用甲醛灭活疫苗免疫儿童(96％曾接种三次以上),随后口服两剂脊髓灰质炎病毒三价活苗,经5周、4年及8年后,采血清检测其中和抗体效价。受检血清保存于-20℃。1968年所采血清曾在1972年重测,并与1972年所采血清同时测定比较。检测是按微量法用猴肾细胞株测

摘要： Diethylpyrocarbonate appears not to react with double-stranded nucleic acids at moderate temperatures, and has been used successfully in the preparation of biologically active double stranded RNA and DNA from bacterial and viral sources. Under conditions of elevated temperature, however, reaction with diethylpyrocarbonate renders poliovirus double-stranded RNA subsequently susceptible to digestion with ribonuclease. Exposure to diethylpyrocarbonate for 10 min at 90° followed by digestion with ribonuclease causes complete conversion to acid-soluble material; the same treatment in the absence of diethylpyrocarbonate causes no degradation of RNA.

关键词： Infectious Disease   Virus Replication   Animal Species   Virus Strain   Vaccine Strain  

摘要： Virus strains proflavin-photosensitized in the course of intracellular synthesis were used to determine the range of animals susceptible to poliovirus infection. Formation of the photoresistant virus population in response to inoculation of photosensitive poliomyelitis virus was observed not only in the CNS of monkeys but also in a number of non-primate animal species. The highest replication activity was shown by the virulent Mahoney strain and rct+ and d+ variants of the LSc2ab strain, judging by the range of susceptible hosts and the titer of virus progeny produced. Replication of vaccine strains was *** conditions excluding virus replication the photosensitivity marker remained stable during contact of the poliovirus-proflavin complex with resistant L cells as it did after inoculation of susceptible animals with guanidine-dependent poliovirus variants.

摘要： Poly(U) isolated from polio [32 P]RF has the base composition (U65 , C2 )Gp. Labeling of the poly(U) in RF with NaIO4 /[3 H]NaBH4 failed, even if the RF was pretreated with phosphatase. Labeling of poly(U) in RF with [γ-32 P]ATP and polynucleotide kinase occurs but only after treatment of the RF with either snake venom exonuclease or polynucleotide phosphorylase. The data suggest that poly(U) is the 5′-end of polio minus RNA.

摘要： Four patients with severe combined immunodeficiency disease developed nonparalytic poliovirus infection after immunization with oral polio vaccine. Allogeneic bone marrow transplantation or administration of unirradiated peripheral lymphocytes induced evidence of graft-versus-host disease in all four of these patients and evidence of immunologic reconstitution in two. These patients stopped excreting poliovirus in their stools shortly after the first evidence of acceptance of the immunocompetent cells. The cells responsible for the immunologic reconstitution of these patients also appear to be responsible for eradicating the poliovirus infection.