关键词： Nucleic Acid Conformation   Base Sequence   Binding Sites   Hela Cells   Humans   Kinetics   Molecular Sequence Data   Neoplasm Proteins   Neuroblastoma   Neurons   Polioviruses   Protein Binding   RNA   Viral   RNA-Binding Proteins   Research Support   Non-U.S. Gov't   Research Support   U.S. Gov't   P.H.S   Ribonucleoproteins   Transcription   Genetic   Tumor Cells   Cultured   Ultraviolet Rays   Uridine  

摘要： Picornavirus RNAs interact with host cellular proteins to direct viral translation initiation by internal ribosome entry. In this study, we analyzed the RNA-protein interactions involving computer-predicted stem-loops F and G (also referred to as V and VI, respectively) of the 5' NCR of poliovirus RNA This region of the 5' NCR harbors part of the putative internal ribosome entry site. We show that a ribonucleoprotein complex involving stem-loop G RNA is composed, at least in part, of a 39-kDa HeLa cell polypeptide which contacts the viral RNA directly. Interestingly, the binding site of a neuronal cell 60-kDa protein, not present in HeLa cells, was mapped specifically to stem-loop G. We also determined that a subset of cellular factors requires a higher order structure synergy before binding to poliovirus RNAs. This was demonstrated by using a longer RNA encompassing both stem-loops F and G in the binding assays. Indeed, a protein with an approximate molecular weight of 36 kDa was shown to interact specifically with these poliovirus sequences. In addition, the role of a cellular polypeptide (p57 or PTB) in poliovirus replication functions was studied. Our results suggest that p57 interactions with stem-loops F-G are not required for internal ribosome binding on poliovirus RNAs. (C) 1995 Academic Press, Inc.

关键词： 脊髓灰质炎病毒   脊灰病毒   野毒株   病毒分离鉴定   急性弛缓性麻痹   中国预防医学科学院   疫苗株   病毒学研究   电泳图像   改进措施  

关键词： 脊髓灰质炎病毒   单克隆抗体   中和试验  

摘要： 本文报告以脊髓灰质炎（以下简称脊灰）病毒SabinⅠ、Ⅱ、Ⅲ型参考株、已定型的阳性标本与相同型别不同毒株特异的单克隆抗体进行微量中和试验。结果表明，我省Ⅰ型分离株中90％为野毒株，Ⅱ型分离株60％为疫苗相关株，40％为野毒株，Ⅱ到分离株50％为疫苗相关株，50％为野毒株，说明我省近年引起脊灰暴发流行仍以Ⅰ型野毒株为主，但Ⅱ、Ⅲ型疫苗株引起的病例也在逐渐增多。

关键词： 川崎病   脊髓灰质炎病毒   发病原因   抗血清   患儿   研究对象   对照组   颊粘膜   研究组   恢复期  

摘要： 作者考虑,川崎病的发病原因似乎跟当时流行的脊髓灰质炎病毒有关,并对此进行了专门的研究,获得了有意义的结果。研究对象是5例川崎病患儿(其中男孩3例,女孩2例,年龄1岁2个月～3岁4个月)。

关键词： 危险因素   肌肉内注射   脊髓灰质炎野病毒   罗马尼亚   VAPP   脊髓灰质炎减毒活疫苗   注射史   服苗病例   免疫   病例对照研究  

摘要： 背景 在罗马尼亚,麻痹型脊髓灰质炎疫苗相关病例(VAPP)的发病率不明原因地比其它国家高5～17倍。很久以前,在脊髓灰质炎野病毒感染的潜伏期期间进行肌肉内注射可以增加麻痹病例发病的危险已引起人们的关注,这一现象也即为“激发性”脊髓灰质炎。为此,作者进行了病例对照研究,以探讨罗马尼亚VAPP与肌肉内注射的关系。 方法 31例VAPP病例均为1988～1992年发病的儿童,其中18例为服苗病例。

关键词： 脊髓灰质炎减毒活疫苗   服用时间   药物   呛咳   简易   使药   正常   呕吐   小儿   观察  

摘要： 脊髓灰质炎减毒活疫苗糖丸(糖丸)规定的服用时间是2～4月婴儿，一般采用溶化后灌服，由于小儿不合作，常造成呛咳或药物溢出，达不到正常药量，同时由于强制性活动，小儿剧烈哭叫，又易引起呕吐，使药物的用量难以估计。为此，我科在近三年发放糖丸时采用口含法服用，经500例观察，认为简便易行。现介绍如下。

关键词： Membrane Proteins   Poliovirus Vaccine   Oral   Vaccines   Attenuated   Virulence   Animals   Brain   Cell Line   Comparative Study   Humans   Macaca fascicularis   Mice   Mice   Transgenic   Poliomyelitis   Polioviruses   Receptors   Virus   Research Support   Non-U.S. Gov't   Spinal Cord   Virus Replication  

摘要： The transgenic mice, ICR-PVRTg21, carrying the human poliovirus receptor gene, were intraspinally inoculated with oral poliovirus vaccine viruses and the viruses that had been derived from a vaccine preparation by passagings at 38 degrees. Dose-dependent incidence of paralysis was observed in the transgenic mice inoculated with any of the viruses used. All transgenic mice showing histopathological lesions in the central nervous system appeared to display clinical signs that resembled those observed in human poliomyelitis. These suggest that relative neurovirulence levels of individual virus preparations can be estimated by Values of 50% paralysis dose. Indeed, the parameter correlated well with monkey lesion scores of viruses used. Histopathological changes such as degeneration of neurons resemble those displayed by monkeys and were observed in the spinal cord, medulla oblongata, and pens. Thus, the transgenic mice, ICR-PVRTg21, may become a new animal model in place of monkeys for safety testing of oral poliovirus vaccine preparations. (C) 1995 Academic Press, Inc.

关键词： 型脊髓灰质炎病毒   小儿散发性脑炎   中枢神经系统   临床表现   肠道病毒   黑龙江省   急性病毒性脑炎   组织培养   血清抗体滴度   上呼吸道感染  

关键词： 疫苗相关麻痹性脊髓灰质炎   危险分析   拉丁美洲   口服脊灰疫苗   调查分析   急性驰缓性麻痹   泛美卫生组织   脊髓灰质炎病毒   专业人员   拉丁美洲国家  

摘要： 近年来在拉丁美洲某些国家里常有疫苗相关麻痹性脊髓灰质炎(VAPP)的报告,此种情况有碍于口服脊灰疫苗(OPV)运动的开展。为查明疫情,泛美卫生组织(PAHO)组织专业人员对各国在1989～1991年报告的6043例急性驰缓性麻痹进行了调查分析。凡是年龄小于15岁。

关键词： 脊髓灰质炎病毒   抗原性   型内差异  

摘要： 对脊髓灰质炎（脊灰）病毒抗原性监测的结果表明，不同年份分离的毒株，其抗原性存在型内差异。对抗ＳａｂｉｎＩ活疫苗血清中和脊灰病毒能力的实验研究发现：（１）要有效地中和流行野毒株，必需高价抗Ｓａｂｉｎ免疫血清；（２）对同一地区同一年份流行的野毒株，中和能力不尽相同；（３）对同一地区流行的野毒株，中和能力随毒株年份的迁延而呈下降趋势。这可能有助于部份解释近年来在脊灰流行中，全程活疫苗免疫者病例有所增加的现象。