关键词： Amino Acid Sequence   Animals   Base Sequence   Centrifugation   Density Gradient   Cesium   Chimeric Proteins   Chlorides   DNA   Viral   Hela Cells   Hepatitis B Core Antigens   Hepatitis B Surface Antigens   Hepatitis B virus   Humans   Mice   Mice   Transgenic   Molecular Sequence Data   Poliovirus   Protein Precursors   Recombination   Genetic   Research Support   Non-U.S. Gov't   Virus Replication  

摘要： Expression of foreign antigens in the context of poliovirus vectors may provide a plausible approach to vaccine development. Poliovirus recombinants were constructed by fusing preS surface or core HBV proteins to the poliovirus polyprotein as previously described (Andino et al., Science, 265, 1448-1451, 1994). All recombinant viruses replicated with near wild-type efficiency in tissue culture cells and stably expressed high levels of the HBV antigens. The kinetics of recombinant RNA synthesis were indistinguishable from that of wild-type poliovirus. Exogenous proteins were not incorporated into the poliovirus particles, but HBV core proteins self-assembled into 100S particles composed of free HBV core proteins and fusions with poliovirus capsid proteins. Mice susceptible to poliovirus infection were inoculated with recombinant virus and elicited humoral immune responses against the HBV antigens. (C) 1996 Academic Press, Inc.

关键词： Viral Proteins   Binding Sites   Cysteine Endopeptidases   Escherichia coli   Hela Cells   Humans   Methionine   Mutagenesis   Site-Directed   Peptides   Polioviruses   Protein Binding   Protein Conformation   Protein Precursors   RNA   Viral   RNA-Binding Proteins   Recombinant Fusion Proteins   Research Support   U.S. Gov't   P.H.S   Substrate Specificity  

摘要： Sequence and structure comparisons with homologous trypsin-like serine proteases have predicted the S1-specificity pocket in picornavirus 3C proteinases. In this study, we examine the putative roles of such residues in poliovirus 3C substrate recognition. Single amino acid substitutions at 3C residues Thr-142, His-161, Gly-163, Gly-164, and Ala-172 were introduced into near full-length poliovirus cDNAs, and protein processing was examined in the context of authentic 3C cis cleavage activity. Our data are consistent with residues Thr-142, His-161, Gly-163, and Gly-164 acting as important determinants of so substrate specificity and support published models of so protein structure. An in vivo analysis of mutant viruses containing individual amino acid substitutions at 3C residues Thr-142 and Ala-172 suggests that such residues are important determinants for viral RNA replication. In addition, bacterially expressed, recombinant 3CD polypeptides containing amino acid substitutions at Thr-142 and Ala-172 show altered RNA binding properties in mobility shift assays that use a synthetic RNA corresponding to the poliovirus 5'-terminal sequences. (C) 1996 Academic Press, inc.

关键词： 蕈状海鞘毒   类似物   疱疹病毒   脊髓灰质炎病毒  

摘要： 应用Hela细胞一病毒感染系统,研究6种合成药对DNA和RNA病毒的增殖抑制作用。结果发现合成药SE4对单纯疱疹病毒1型(HSV-1)和脊髓灰质炎病毒都有抑制作用,抑制浓度分别为1.56μg/ml和25μg/ml。当浓度为25μg/ml时,SE4对HSV-1具有明显杀伤作用,可完全抑制该病毒的增殖。实验结果表明,合成药SE4对DNA病毒的作用优于RNA病毒。

关键词： 脊髓灰质炎病毒   疫苗株   循环动态   环境水  

摘要： 1993～1995年作者在连云港市选近三年曾有脊髓灰质炎病例的乡(镇),采用氯化钠—三氯化铝沉淀法对河塘污水中脊髓灰质炎病毒(PV)循环动态进行研究。发现:在连续二年的各个季节采集的水样中均能分离到致Hep-2细胞病变的病毒(57.95%),经鉴定:21株为PV(23.86%),其中PV Ⅰ 18株、PV Ⅱ 1株、PV Ⅲ 2株,另30株(34.09%)为非脊灰肠道病毒(NPEV)。鉴于我国目前脊灰流行毒株主要为PV Ⅰ,经RT-PCR试验,所分离的PV Ⅰ均为疫苗(Sabin)相关株,初步表明调查地区的外环境的PV已被疫苗相关株所取代。

关键词： 脊髓灰质炎   婴儿   病毒  

摘要： 河南省四县市健康婴儿脊髓灰质炎病毒带毒情况调查谢秋莲，徐瑾，李延军，陈美光，崔长法，贾庆梅（河南省卫生防疫站，郑州市，４５０００３）为了了解脊髓灰质炎（脊灰）在高危人群中的循环动态，尤其是６月龄以内未接受三价口服脊灰活疫苗（ＴＯＰＶ）免疫的婴儿携带脊...

关键词： 腹泻   脊髓灰质炎疫苗   血清转化   脊髓灰质炎病毒   接种   血清标本   婴幼儿   口服   年龄相关   抗体滴度  

摘要： 本研究目地是了解腹泻对口服三联脊髓灰质炎疫苗(OPV)血清转化的影响。6岁～16周的婴幼儿分别于试验开始。第4周和第8周接受了单剂OPV,并设了一年龄相关的对照组。在试验起始,第2剂之前和经3剂之后采了血清标本,通过微中和法测定了抗体滴度。结果为:第一次接种后,腹泻者对脊髓灰质炎病毒2型和3型的血清反应低于对照组。分别为26和34％(P<0.002)。第3次接种后。

关键词： CO2激光   脊髓灰质炎疫苗   尖锐湿疣   激光疗法   治疗方法   CA  

摘要： 我们于1993年11月至95年3月间,应用CO_2激光和大剂量脊髓灰质炎减毒活疫苗Ⅰ、Ⅱ、Ⅲ型混合三价糖丸治疗多次复发性尖锐湿疣25例,收到满意效果,现报告如下。