关键词:
ANOXEMIA
HYPERTENSION
TACHYCARDIA
BAROREFLEXES
BRADYCARDIA
AVERTIN
MICE as laboratory animals
摘要:
Chronic intermittent hypoxia (CIH) induces systemic hypertension and tachycardia, and impairs baroreflex bradycardia in Avertin anesthetized mice (Lin et al. 2006). However, anesthesia significantly blunts phenylephrine (PE)- and completely abolishes sodium nitroprusside (SN)-induced heart rate response. In this study, we studied baroreflex sensitivity in conscious mice. Mice were exposed to either room air (RA) or CIH (6 min alternations of 21% O2 and 5.7 % O2) starting at the age of 3 months for 4 months. Mean arterial blood pressure (mABP) and mean heart rate (mHR) were measured from a femoral artery catheter. PE and SN were administered using a microinfusion pump (100 µg/ml; 5, 10, 20, 30, and 40 µl/min i.v.) for 1 min. Changes in mHR (ΔmHR) were plotted against changes in mABP (ΔmABP). Our data indicate that (1) Resting mABP and mHR baseline were significantly higher in CIH mice (CIH: 98.5 ± 1.0 mmHg, 611.5 ± 11.1 bmp, n=18 vs. RA: 92.1 ± 3.1 mmHg, 519.2 ± 30.6 bmp; n = 15; P< 0.05). (2) Baroreflex sensitivity (ΔHR / ΔmBAP) in RA conscious mice is markedly greater than in Avertin anesthetized RA mice (conscious: -4.4 ± 0.5beats / mmHg vs. anesthetized: -0.32 ± 0.06 beats / mmHg in SN induced-baroreflex tachycardia, n=10, p<0.01; conscious: -12.1 ± 0.9 beats / mmHg vs. anesthetized: -5.8 ± 0.3 beats / mmHg in PE induced-baroreflex bradycardia, n=10, p<0.01). (3) CIH significantly decreases PE- and SN-induced baroreflex heart rate responses (two-way ANOVA, p<0.01). We conclude that CIH induces systemic hypertension and relative tachycardia, and impairs of baroreflex control of heart rate in conscious mice. [ABSTRACT FROM AUTHOR]